donor stock number: E387
NASC stock number: N70031
Resource Type: seed
Availability: available
Donors:- Scott Poethig
Donation Date: 11/12/2002
Date Released: 11/18/2002
Description:
Enhancer trap T-DNA line transformed with vector pBIN m-gfp5-ER; this vector is derived from pBI121; contains a BAMH1-SST1 fragment carrying a modified GFP gene - a cryptic intron has been eliminated, endoplasmic reticulum (ER) retention signals have been incorporated and mutated to allow improved folding of the GFP at 37C in E. coli and mutated to allow visualization at UV (395nm) or blue light (473nm); the m-gfp5-ER gene is GAL4 responsive; kanamycin resistant.
Growth Requirement: none
Marker: kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pBIN m-gfp5-ER | enhancer trap T-DNA derived from pBI121; contains a BAMH1-SST1 fragment carrying a modified GFP gene with ER retention singnal | GFP | kanamycin |
Quality Control Comments
| Comment | Commented by | Date | |
|---|---|---|---|
| Germination was found to be 38%; this stock will be replanted to increase germination. Until the stock is replanted an adjusted higher amount of seeds will be sent for this stock. | ABRC | 03/10/2015 | To view detailed data, click here |
| researcher reported low germination | ABRC | 03/09/2015 | To view detailed data, click here |
| Previous low germination stock was replaced with a stock that the germination was found to be 96%. | ABRC | 04/06/2016 | To view detailed data, click here |