donor stock number: E3700
NASC stock number: N70216
Resource Type: seed
Availability: available
Donors:- Scott Poethig
Donation Date: 06/11/2003
Date Released: 07/01/2003
Description:
Enhancer trap T-DNA line transformed with vector pBIN m-gfp5-ER; this vector is derived from pBI121; contains a BAMH1-SST1 fragment carrying a modified GFP gene - a cryptic intron has been eliminated, endoplasmic reticulum (ER) retention signals have been incorporated and mutated to allow improved folding of the GFP at 37C in E. coli and mutated to allow visualization at UV (395nm) or blue light (473nm); the m-gfp5-ER gene is GAL4 responsive; kanamycin resistant
Growth Requirement: none
Marker: kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
homozygous
T-DNA insertion
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pBIN m-gfp5-ER | enhancer trap T-DNA derived from pBI121; contains a BAMH1-SST1 fragment carrying a modified GFP gene with ER retention singnal | GFP | kanamycin |
Quality Control Comments
There is no quality control data for this stock.