other name: LRC1-4
donor stock number: JYB234.1
NASC stock number: N70508
Resource Type: seed
Availability: available
Donors:- Philip Benfey
- Ji-Young Lee
Donation Date: 10/30/2007
Date Released: 08/13/2008
Description:
Transgenic GFP-fusion expression line harboring the pLRC1-4(AT1G08930)::ER-GFP:NOS construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Growth Requirement: Segregating for the transgene. basta resistant.
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Transcription factor (TF) expressed in a tissue-enriched manner in roots: patchy expression in epidermis from early elongation zone (5/5; 6DFG); there is strong expression in cortex from maturation zone (3/5; 6DAG; 4.5% sucrose), same in 1% sucrose.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1073/pnas.0510607103 | 16581911 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pLRC1-4::ER-GFP:NOS | GFP-fusion expression construct | GFP | BASTA |
Quality Control Comments
There is no quality control data for this stock.