NASC stock number: N71602
donor stock number: p35S:ARR1deltaDDK:GR
Resource Type: seed
Availability: available
Donors:- Joseph Ecker
- Mingtang Xie
Donation Date: 06/18/2018
Date Released: 07/12/2018
Description:
Transgenic line. The active form of ARR1 was constructed using a deletion of the receiver domain of ARR1. This ARR1deltaDDK deletion construct was fused at the C-terminus to the glucocorticoid-inducible artificial transcription factor (GR), cloned into pENTR/D-TOPO, and then recombined into pEarleyGate104 to be in frame of an N-terminal YFP reporter. Col-0 plants were transformed by the floral dip method. Basta resistant. Homozygous for the transgene.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Dexamethasone (Dex)-inducible cytokinin hyper-response phenotype. No visible phenotype without Dex induction.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1038/s41467-018-03921-6 | 29686312 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| p35S:ARR1deltaDDK:GR | The active form of ARR1 was constructed using a deletion of the receiver domain of ARR1. This ARR1deltaDDK deletion construct was fused at the C-terminus to the glucocorticoid-inducible artificial transcription factor (GR), cloned into pENTR/D-TOPO, and then recombined into pEarleyGate104 to be in frame of an N-terminal YFP reporter. | CaMV 35S | YFP | BASTA |
Quality Control Comments
There is no quality control data for this stock.