NASC stock number: N71613
other name: camta2 camta3 CAMTA3p:CAMTA3 (A855V)-GFP line 27
donor stock number: AV27
Resource Type: seed
Availability: available
Donors:- Sarah Gilmour
- Michael Thomashow
Donation Date: 06/19/2018
Date Released: 07/17/2018
Description:
Transgenic line expressing CAMTA3-GFP protein variant CAMTA3 (A855V) in a camta2 camta3 double mutant background. The mutated CAMTA3 sequence was recombined into vector pEGC3PGFP, a binary vector based on pEarleyGate100, but containing the CAMTA3 endogenous promoter (2-kb) and an in-frame terminal GFP derived from pEarleyGate103. camta2 camta3 double mutant (CS71604) plants were transformed by the floral dip method. Basta resistant. Homozygous for the transgene.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Severely impaired cold induction of salicylic acid (SA) pathway genes. Compromised immunity against bacterial pathogen Pst DC3000.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1105/tpc.16.00865 | 28982964 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pROK2 | T-DNA | kanamycin | ||
| CAMTA3p:CAMTA3 (A855V)-GFP | CAMTA3-GFP protein variant CAMTA3 (A855V). The mutated CAMTA3 sequence was recombined into vector pEGC3PGFP, a binary vector based on pEarleyGate100, but containing the CAMTA3 endogenous promoter (2-kb) and an in-frame terminal GFP derived from pEarleyGate103. | CAMTA3 | GFP | BASTA |
Quality Control Comments
There is no quality control data for this stock.