ABRC
donor stock number: PIF1-OE1 CTG10-OE2
NASC stock number: N71652
other name: ABD239
Resource Type: seed
Availability: available
Donors:- Bruce Downie
Donation Date: 07/19/2018
Date Released: 09/07/2018
Description:
Transgenic line overexpressing PIF1 and CTG10. Generated by crossing PIF1-OE1 (from Giltsu Choi) to CTG10-OE2 (CS71629). Kanamycin and basta resistant. Homozygous for the transgenes.
Growth Requirement: none
Marker: BASTA, kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
Ploidy: 2
Phenotype: Repression of seed germination mediated by PIF1 overexpression is partially alleviated by the concurrent overexpression of CTG10.
Taxon: Arabidopsis thaliana 3702
Publications
| DOI | PubMed |
|---|---|
| 10.1073/pnas.1711919115 | 29632208 |
Transgenes
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| PIF1-OE | PIF1 overexpression construct | CaMV 35S | kanamycin | |
| CTG10-OE | full-length CTG10 with dual CaMV 35S promoters and Tobacco etch virus translational enhancer | CaMV 35S | BASTA |
Quality Control Comments
There is no quality control data for this stock.