Name / Stock Number: CS71685

donor stock number: SOMA-NES-1

NASC stock number: N71685

Resource Type: seed

Availability: available

Donors:
  • Patrick Krysan

Donation Date: 10/12/2018

Date Released: 11/29/2018

Description:

Transgenic line expressing SOMA (sensor of MAPK activity) with a nuclear exclusion signal. For targeted expression of the sensor in the cytoplasm of transgenic plants, the SOMA protein coding sequence was moved into a derivative of the binary vector pRI 201-AN (Takara Bio, USA) to produce pSOMA-NES. Col-0 plants were transformed by the floral dip method and the transgenic line was identified by screening T1 seed for seedlings showing YFP fluorescence. Genotype of the transgene is unknown.

Growth Requirement: This T-DNA line expresses a FRET sensor for monitoring MAP kinase activity in Arabidopsis. Because gene silencing can affect the expression of the FRET sensor, one should always screen young seedlings on agar plates for YFP or CFP fluorescence to identify individual seedlings in which the sensor is being properly expressed before doing an imaging experiment. When bulking seeds for these lines, one should also screen the individual seedlings used to bulk up seed for YFP or CFP fluorescence.

Marker: kanamycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

No visible phenotype

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.1111/tpj.14164 30444549
Transgene Description Promoter Reporter Marker
pSOMA-NES SOMA (sensor of MAPK activity) with a nuclear exclusion signal CaMV 35S YPet;Turquoise-GL kanamycin

Quality Control Comments

There is no quality control data for this stock.