donor stock number: MPK6YG SOMA-NES
NASC stock number: N71691
Resource Type: seed
Availability: available
Donors:- Patrick Krysan
Donation Date: 10/12/2018
Date Released: 11/29/2018
Description:
Transgenic line expressing SOMA (sensor of MAPK activity) with a nuclear exclusion signal in the mpk3 mpk6 PMPK6:MPK6YG (MPK6SR) genetic background. Generated by genetic crossing of SOMA-NES-1 (CS71685) to the MPK6SR31 line (Xu et al., 2014). Homozygous for the mpk3-1 and mpk6-2 mutations. Homozygous for the PMPK6:MPK6YG transgene; genotype of the pSOMA-NES transgene is unknown.
Growth Requirement: This T-DNA line expresses a FRET sensor for monitoring MAP kinase activity in Arabidopsis. Because gene silencing can affect the expression of the FRET sensor, one should always screen young seedlings on agar plates for YFP or CFP fluorescence to identify individual seedlings in which the sensor is being properly expressed before doing an imaging experiment. When bulking seeds for these lines, one should also screen the individual seedlings used to bulk up seed for YFP or CFP fluorescence.
Marker: BASTA, kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
No visible phenotype
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1111/tpj.14164 | 30444549 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pSOMA-NES | SOMA (sensor of MAPK activity) with a nuclear exclusion signal | CaMV 35S | YPet;Turquoise-GL | kanamycin |
| PMPK6:MPK6YG | MPK6 native promoter driven MPK6 cDNA with mutated Tyr144 residue (to Glyin) in the ATP binding pocket | MPK6 | BASTA | |
| pROK2 | T-DNA | kanamycin |
Quality Control Comments
There is no quality control data for this stock.