donor stock number: 25:26
NASC stock number: N71719
Resource Type: seed
Availability: available
Donors:- Antonius Matzke
- Marjori Matzke
Donation Date: 11/26/2018
Date Released: 12/19/2018
Description:
Chromosome 2 fluorescence-tagged tetraploid transgenic line expressing construct 25, an enhanced GFP and lac repressor/operator system, and a second construct containing the RPS5 promoter to drive expression of genes encoding the EGFP-LacI fusion protein (GL) and histone H2B fused to monomeric DsRed (H2BmR) (Fig. 1C, construct 5; Matzke et al., 2010). Col-0 plants were transformed by the floral dip method. Partially resistant to kanamycin. Phosphinotricin resistant. Homozygous for the transgenes. Tetraploid plants were produced by colchicine treatment.
Growth Requirement: none
Marker: phosphinothricin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
4
No visible phenotype
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| Construct 25 | construct containing an enhanced GFP and lac repressor/operator system | CaMV 35S | EGFP | kanamycin |
| Construct C5 | construct containing the RPS5 promoter to drive expression of genes encoding the EGFP-LacI fusion protein (GL) and histone H2B fused to monomeric DsRed (H2BmR) | RPS5 | EGFP;DsRed | phosphinothricin |
Quality Control Comments
There is no quality control data for this stock.