NASC stock number: N71744
other name: TREPH1S625A/ treph1-1
other name: F-625A
donor stock number: dPro35S::His::YFP::TREPH1S625A/treph1-1
Resource Type: seed
Availability: available
Donors:- Ning Li
Donation Date: 12/12/2018
Date Released: 01/08/2019
Description:
treph1-1 mutant expressing dPro35S::His::YFP::TREPH1S625A. Point-mutated TREPH1S625A was generated from genomic DNA using mutagenic reverse primers, fused with a His-YFP tag at the N terminus and ligated to binary vector pHub10 for expression under the control of a double CaMV 35S promoter. treph1-1 (SALK_023690C) mutant plants were transformed by the floral dip method. Hygromycin resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
No delay in bolting in response to touch.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1073/pnas.1814006115 | 30291188 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| dPro35S::His::YFP::TREPH1S625A | point-mutated TREPH1S625A fused with a His-YFP tag at the N terminus under the control of a double CaMV 35S promoter | 2x CaMV 35S | His-tag; YFP | hygromycin |
| pROK2 | T-DNA | kanamycin |
Quality Control Comments
There is no quality control data for this stock.