Name / Stock Number: CS71750

NASC stock number: N71750

donor stock number: 35S:GNC-L1

Resource Type: seed

Availability: available

Donors:
  • Yan Zubo
  • G. Schaller

Donation Date: 01/14/2019

Date Released: 02/22/2019

Description:

Transgenic line overexpressing GNC. The genomic sequence of GNC was amplified from Col-0 genomic DNA and cloned into pCR8/GW/TOPO to generate an entry clone. The GNC entry clone was recombined into pEarleyGate 103 for C-terminal fusion with GFP and expression driven by the CaMV 35S promoter. Col-0 plants were transformed by the floral dip method and selection was performed based on resistance to Basta. Homozygous for the transgene.

Growth Requirement: none

Marker: BASTA

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

Dark-green leaves, reduced shoot growth, small rosette, slight reduction in rosette leaf number. Enhanced chloroplast production in the roots and in both epidermal pavement and stomatal guard cells. Delayed bolting. Increased NPQ (nonphotochemical energy quenching) and a decrease in the flux of electrons through PSII. Increased stomatal and meristemoid indexes.

Arabidopsis thaliana 3702

Additional Information

Transgene Description Promoter Reporter Marker
35S:GNC-GFP genomic sequence of GNC driven by the CaMV 35S promoter, C-terminal fusion with GFP CaMV 35S GFP BASTA

Quality Control Comments

There is no quality control data for this stock.