NASC stock number: N71756
donor stock number: 35S:GNC/GLK2-L3
Resource Type: seed
Availability: available
Donors:- Yan Zubo
- G. Schaller
Donation Date: 01/14/2019
Date Released: 02/22/2019
Description:
Transgenic line overexpressing GNC and GLK2. Agrobacterium strain GV3101, transformed with the GLK2 construct in pGWB405 (35S:GLK2-GFP), was used to transform 35S:GNC plants by the floral dip method. Selection was performed based on resistance to Basta (GNC) or kanamycin (GLK2). Homozygous for the transgenes.
Growth Requirement: none
Marker: kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Small rosette, severe growth reduction in the shoot, and delayed maturation. Chlorotic interveinal spaces, dark green veinal regions, enhanced chloroplast production in the roots. Reduced sizes of mesophyll chloroplasts and increased size and number of chloroplasts in stomatal guard cells. Significant decreases in photosynthetic parameters related to the quantum yield of PSII and both photochemical and nonphotochemical quenching.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1104/pp.18.00414 | 30002259 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| 35S:GNC-GFP | genomic sequence of GNC driven by the CaMV 35S promoter, C-terminal fusion with GFP | CaMV 35S | GFP | BASTA |
| 35S:GLK2-GFP | genomic sequence of GLK2 driven by the CaMV 35S promoter, C-terminal fusion with GFP | CaMV 35S | GFP | kanamycin |
Quality Control Comments
There is no quality control data for this stock.