Name / Stock Number: CS71756

NASC stock number: N71756

donor stock number: 35S:GNC/GLK2-L3

Resource Type: seed

Availability: available

Donors:
  • Yan Zubo
  • G. Schaller

Donation Date: 01/14/2019

Date Released: 02/22/2019

Description:

Transgenic line overexpressing GNC and GLK2. Agrobacterium strain GV3101, transformed with the GLK2 construct in pGWB405 (35S:GLK2-GFP), was used to transform 35S:GNC plants by the floral dip method. Selection was performed based on resistance to Basta (GNC) or kanamycin (GLK2). Homozygous for the transgenes.

Growth Requirement: none

Marker: kanamycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

Small rosette, severe growth reduction in the shoot, and delayed maturation. Chlorotic interveinal spaces, dark green veinal regions, enhanced chloroplast production in the roots. Reduced sizes of mesophyll chloroplasts and increased size and number of chloroplasts in stomatal guard cells. Significant decreases in photosynthetic parameters related to the quantum yield of PSII and both photochemical and nonphotochemical quenching.

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.1104/pp.18.00414 30002259
Transgene Description Promoter Reporter Marker
35S:GNC-GFP genomic sequence of GNC driven by the CaMV 35S promoter, C-terminal fusion with GFP CaMV 35S GFP BASTA
35S:GLK2-GFP genomic sequence of GLK2 driven by the CaMV 35S promoter, C-terminal fusion with GFP CaMV 35S GFP kanamycin

Quality Control Comments

There is no quality control data for this stock.