NASC stock number: N71760
donor stock number: 35S:KMD1-11
Resource Type: seed
Availability: available
Donors:- G. Schaller
Donation Date: 01/14/2019
Date Released: 02/22/2019
Description:
Transgenic line overexpressing KMD1. cDNA covering the complete KMD1 coding sequence was cloned into pCR8/GW/TOPO and recombined into pEarleyGate 103 for C-terminal fusion with GFP and expression driven by the CaMV 35S promoter. Col-0 plants were transformed by the floral dip method and selection was performed based on resistance to Basta. Homozygous for the transgene.
Growth Requirement: none
Marker: BASTA
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Cytokinin hyposensitivity. Increased size of root meristem.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1073/pnas.1300403110 | 23720308 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| 35S:KMD1 | complete KMD1 coding sequence driven by the CaMV 35S promoter, C-terminal fusion with GFP | CaMV 35S | GFP | BASTA |
Quality Control Comments
There is no quality control data for this stock.