NASC stock number: N71807
other name: tETR1-H/G2/D #2 (etr1-9;ers1-3)
donor stock number: tETR1-H/G2/D #2
Resource Type: seed
Availability: available
Donors:- G. Schaller
Donation Date: 02/18/2019
Date Released: 03/26/2019
Description:
Transgenic expression of kinase-inactive ETR1 (tETR1-H/G2/D) in the etr1-9;ers1-3 double mutant background. The ETR1-H/G2 genomic clone was subcloned into pALTERII and the Altered Sites Mutagenesis System was used for site-directed mutation of the conserved Asp residue of the receiver domain to an Asn residue (Asp-659Asn). The mutant product was then cloned into binary vector pCAMBIA2380. Since the etr1-9;ers1-3 homozygous double mutant is sterile, the construct was initially transformed into etr1-9/etr1-9;ers1-3/ERS1 plants, and plants homozygous for the mutations and the transgene were identified in subsequent generations. Kanamycin resistant.
Growth Requirement: none
Marker: kanamycin
Background: Ws (Wassilewskija)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Ethylene hyposensitive.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1104/pp.112.196790 | 22467798 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| tETR1-H/G2/D | kinase-inactive ETR1 (tETR1-H/G2/D) generated from a genomic clone by site-directed mutation of the conserved Asp residue of the receiver domain to an Asn residue (Asp-659Asn). | ETR1 | kanamycin | |
| pSKI15 | activation tag T-DNA | 4x CaMV 35S | BASTA |
Quality Control Comments
There is no quality control data for this stock.