Name / Stock Number: CS71814

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NASC stock number: N71814

other name: ERS2-TAP (ers2-3)

donor stock number: ERS2-TAP

Resource Type: seed

Availability: available

Donors:
  • G. Schaller

Donation Date: 02/18/2019

Date Released: 03/26/2019

Description:

ers2-3 mutant expressing ERS2 driven by its native promoter with C-terminal streptaviden-binding peptide (SBP) and tandem affinity purification (TAP) tags. The region encoding ERS2 along with the upstream promoter sequence was amplified from the Arabidopsis BAC clone F19P19 (GenBank accession no. AC000104) and the PCR product was cloned into vector pCAMBIA1380-SBP-TAP. The construct was transformed into the corresponding single loss-of-function mutant background, ers2-3. Hygromycin B resistant. Homozygous for the transgene.

Growth Requirement: none

Marker: hygromycin

Background: Ws (Wassilewskija)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Genes

Gene Names Evidence
AT1G04310
  • ERS2
  • ethylene response sensor 2
    		•  polymorphism SIGnAL TAIR ARAPORT

    Germplasm

    2

    No visible phenotype.

    Polymorphisms

    Polymorphism Mutagen Genotype Inheritance Gene Evidence attribution
    ers2-3 T-DNA insertion homozygous recessive
  • AT1G04310

    • Arabidopsis thaliana 3702

    Additional Information

    DOI PubMed
    10.1074/jbc.M800641200 18577522
    Transgene Description Promoter Reporter Marker
    3850:1003 T-DNA kanamycin
    ERS2-TAP ERS2 driven by its native promoter with C-terminal streptaviden-binding peptide (SBP) and tandem affinity purification (TAP) tags ERS2 hygromycin

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