NASC stock number: N72204
donor stock number: pRIN4::T7-gRIN4T166D rpm1-3 rps2-101c rin4 #1
Resource Type: seed
Availability: available
Donors:- Jeff Dangl
Donation Date: 05/22/2019
Date Released: 07/09/2019
Description:
rpm1-3 rps2-101c rin4 triple mutant (CS68760) expressing a T7-tagged phosphomimic T166D mutant of RIN4 driven by the native RIN4 promoter. The transgene was cloned into the pBAR1-GW destination vector for Basta selection and plant transformation was performed using the floral dip method. Potentially segregating for the transgene.
Growth Requirement: none
Marker: BASTA
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Suppression of callose accumulation upon flg22 treatment. Weak enhanced disease susceptibility (EDS) phenotype against P. syringae virulence strain DC3000.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1016/j.chom.2014.09.004 | 25299334 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pRIN4::T7-gRIN4T166D | T7-tagged T166D phosphomimic AvrB binding site (BBS) mutant of RIN4 driven by the native RIN4 promoter | RIN4 | BASTA | |
| pROK2 | T-DNA | kanamycin |
Quality Control Comments
There is no quality control data for this stock.