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Name / Stock Number: CS72221

OPT3p::LUC line 3-9

N72221

seed

available

  • David Mendoza-Cozatl
  • 06/14/2019

    07/16/2019

    Description:

    Transgenic line expressing the firefly luciferase reporter gene under the control of the OPT3 promoter. The 3.0 kb sequence upstream from the start codon of OPT3 was cloned into the pENTR/D-TOPO entry vector. The insert was then transferred to a modified pGreenII 0179 plasmid containing the Luciferase coding sequence. Presence of the insert was confirmed by colony PCR and plasmid sequencing. Col-0 plants were transformed by the floral dip method. Hygromycin resistant. Homozygous for the transgene.

    none

    hygromycin

    Col (Columbia)

    100 seeds per vial

    $10 / $80

    Germplasm

    2

    Luciferase expression induced by Fe-deficient conditions or luciferin treatment.

    Additional Information

    OPT3p::LUC

    OPT3

    LUC

    hygromycin

    Arabidopsis thaliana 3702

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