NASC stock number: N72231
donor stock number: ProRUBY:RUBY-Citrine
Resource Type: seed
Availability: available
Donors:- George Haughn
Donation Date: 06/25/2019
Date Released: 07/23/2019
Description:
mum2-1 ruby-1 double mutant complemented with the ProRUBY:RUBY-Citrine transgene. The RUBY coding sequence and 4477 bp upstream sequence containing regulatory elements (RUBY promoter) were cloned into the pCAMBIA2300 plasmid along with a gene encoding the Citrine fluorescent tag and the nopaline synthase terminator sequence. mum2-1 ruby-1 (CS72223) plants were transformed by the floral dip method and kanamycin resistant plants were selected on AT agar medium. Genotype of the transgene is unknown.
Growth Requirement: select on kanamycin plates
Marker: kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
No seed coat mucilage extrusion in water.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1105/tpc.18.00954 | 30852555 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| ProRUBY:RUBY-Citrine | RUBY coding sequence and 4477 bp upstream sequence containing regulatory elements (RUBY promoter) with a gene encoding the Citrine fluorescent tag and the nopaline synthase terminator sequence | RUBY | Citrine | kanamycin |
Quality Control Comments
There is no quality control data for this stock.