Name / Stock Number: CS72240

NASC stock number: N72240

other name: elf3-13 PRR7::PRR7-LUC

donor stock number: elf3-13

Resource Type: seed

Availability: available

Donors:
  • Yeon Jeong Kim
  • David Somers

Donation Date: 07/03/2019

Date Released: 08/20/2019

Description:

elf3-13 mutant identified from a forward genetic screen for PRR7 protein turnover factors. EMS mutagenesis was performed on a transgenic line expressing a PRR7::PRR7-luciferase translational fusion. To generate the transgenic line, the PRR7 coding sequence was subcloned into a pPZP-BAR DONR plasmid harboring luciferase fused to 1208 bp of the PRR7 promoter. A genomic fragment containing 2223 bp upstream of the 5 end of PRR7 was then cloned upstream of the PRR7 gene to replace the 1208 bp promoter. Col-0 plants were transformed by the floral dip method. Basta resistant. Homozygous for the transgene; presumed homozygous for the mutation.

Growth Requirement: none

Marker: BASTA

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

Early flowering, long hypocotyl.

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.3389/fpls.2019.00667 31191580
Transgene Description Promoter Reporter Marker
PRR7::PRR7-LUC PRR7::PRR7-luciferase translational fusion PRR7 LUC BASTA

Quality Control Comments

There is no quality control data for this stock.