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Name / Stock Number: CS72243

ss1551

N72243

seed

not available

  • Steve Jacobsen
  • 07/17/2019

    08/20/2019

    Description:

    Transgenic line expressing self-resistance gene astD. A 35-amino acid residue chloroplast localization signal (CLS) derived from the endogenous A. thaliana DHAD gene was fused to the N-terminus of an A. terreus astD gene codon-optimized for A. thaliana, and a 3xFLAG-tag was inserted in between. The construct was cloned into a pEG202 vector modified to express the transgene from the UBQ10 promoter. Col-0 plants were transformed by the floral dip method. BASTA and aspterric acid resistant. Segregating for the transgene. Independent transgenic line 1; original donated stock is from the T2 generation.

    Positive transgenic plants can be selected with glufosinate and testing for survival in the presence of aspterric acid.

    BASTA

    Col (Columbia)

    100 seeds per vial

    $10 / $80

    Germplasm

    2

    Additional Information

    pUB10:CLS35-AstD

    UBQ10 promoter driving expressing of a transgene composed of a 35-amino acid residue chloroplast localization signal (CLS) derived from the endogenous A. thaliana DHAD gene fused to the N-terminus of an A. terreus astD gene codon-optimized for A. thaliana, with a 3xFLAG-tag inserted in between.

    UBQ10

    BASTA

    Arabidopsis thaliana 3702

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