Name / Stock Number: CS72243
Transgenic line expressing self-resistance gene astD. A 35-amino acid residue chloroplast localization signal (CLS) derived from the endogenous A. thaliana DHAD gene was fused to the N-terminus of an A. terreus astD gene codon-optimized for A. thaliana, and a 3xFLAG-tag was inserted in between. The construct was cloned into a pEG202 vector modified to express the transgene from the UBQ10 promoter. Col-0 plants were transformed by the floral dip method. BASTA and aspterric acid resistant. Segregating for the transgene. Independent transgenic line 1; original donated stock is from the T2 generation.
Positive transgenic plants can be selected with glufosinate and testing for survival in the presence of aspterric acid.
100 seeds per vial
$10 / $80
UBQ10 promoter driving expressing of a transgene composed of a 35-amino acid residue chloroplast localization signal (CLS) derived from the endogenous A. thaliana DHAD gene fused to the N-terminus of an A. terreus astD gene codon-optimized for A. thaliana, with a 3xFLAG-tag inserted in between.
Arabidopsis thaliana 3702
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