Name / Stock Number: CS72243

NASC stock number: N72243

donor stock number: ss1551

Resource Type: seed

Availability: available

Donors:
  • Steve Jacobsen

Donation Date: 07/17/2019

Date Released: 08/20/2019

Description:

Transgenic line expressing self-resistance gene astD. A 35-amino acid residue chloroplast localization signal (CLS) derived from the endogenous A. thaliana DHAD gene was fused to the N-terminus of an A. terreus astD gene codon-optimized for A. thaliana, and a 3xFLAG-tag was inserted in between. The construct was cloned into a pEG202 vector modified to express the transgene from the UBQ10 promoter. Col-0 plants were transformed by the floral dip method. BASTA and aspterric acid resistant. Segregating for the transgene. Independent transgenic line 1; original donated stock is from the T2 generation.

Growth Requirement: Positive transgenic plants can be selected with glufosinate and testing for survival in the presence of aspterric acid.

Marker: BASTA

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.1038/s41586-018-0319-4 29995859
Transgene Description Promoter Reporter Marker
pUB10:CLS35-AstD UBQ10 promoter driving expressing of a transgene composed of a 35-amino acid residue chloroplast localization signal (CLS) derived from the endogenous A. thaliana DHAD gene fused to the N-terminus of an A. terreus astD gene codon-optimized for A. thaliana, with a 3xFLAG-tag inserted in between. UBQ10 BASTA

Quality Control Comments

There is no quality control data for this stock.