Name / Stock Number: CS72456

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NASC stock number: N72456

donor stock number: 35S::AGB1 R25D E248K line 3-2D

Resource Type: seed

Availability: available

Donors:
  • Alan Jones

Donation Date: 02/01/2021

Date Released: 03/12/2021

Description:

Transgenic line stably expressing AGB1 variant in the Gbeta-null agb1-2 mutant background. The binary construct in the pGWB backbone was introduced into Agrobacterium tumefaciens by electroporation and then into the agb1-2 mutant (CS6536) by floral dipping. Hygromycin resistant. Homozygous for the transgene.

Growth Requirement: none

Marker: hygromycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Genes

Gene Names Evidence
AT4G34460
  • ATAGB1
  • AGB1
  • ELK4
  • GTP binding protein beta 1
  • ERECTA-LIKE 4
    		•  transgene SIGnAL TAIR ARAPORT

    Germplasm

    2

    Partial complementation of the agb1-2 lateral root, fungal pathogen hypersusceptibility, and flg22-triggered ROS formation phenotypes.

    Polymorphisms

    Polymorphism Mutagen Genotype Inheritance Gene Evidence attribution
    agb1-2 T-DNA insertion homozygous recessive
  • AT4G34460
    		• TAIR

    Arabidopsis thaliana 3702

    Additional Information

    DOI PubMed
    10.1104/pp.112.196337 22570469
    Transgene Description Promoter Reporter Marker
    35S::AGB1 R25D E248K AGB1 variant generated through site-directed mutagenesis expressed under the control of the 35S promoter CaMV 35S hygromycin

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