Name / Stock Number: CS72458

NASC stock number: N72458

donor stock number: 35S::AGB1 W109A line 4-4C

Resource Type: seed

Availability: available

Donors:
  • Alan Jones

Donation Date: 02/01/2021

Date Released: 03/12/2021

Description:

Transgenic line stably expressing AGB1 variant in the Gbeta-null agb1-2 mutant background. The binary construct in the pGWB backbone was introduced into Agrobacterium tumefaciens by electroporation and then into the agb1-2 mutant (CS6536) by floral dipping. Hygromycin resistant. Homozygous for the transgene.

Growth Requirement: none

Marker: hygromycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

Partial complementation of the agb1-2 hypocotyl, lateral root, and ABA-hypersensitivity phenotypes. Complete rescue of agb1-2 scorable leaf, silique, fungal pathogen hypersusceptibility, and flg22-triggered ROS formation phenotypes.

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.1104/pp.112.196337 22570469
Transgene Description Promoter Reporter Marker
35S::AGB1 W109A AGB1 variant generated through site-directed mutagenesis expressed under the control of the 35S promoter CaMV 35S hygromycin

Quality Control Comments

There is no quality control data for this stock.