Name / Stock Number: CS72511

NASC stock number: N72511

donor stock number: HSP101-PP7-2.1

Resource Type: seed

Availability: available

Donors:
  • Krishna Niyogi

Donation Date: 04/06/2021

Date Released: 06/02/2021

Description:

Transgenic line. Col-0 plants were transformed with the UPG plasmid (Addgene #161003) coding for a PCP-mGFP5 fusion driven by the Arabidopsis UBQ10 constitutive promoter and a second construct containing a PP7 reporter driven by the Arabidopsis HSP101 promoter and a H2B-mScarlet nuclear marker (based on Addgene plasmid #161006). Hygromycin and kanamycin resistant. Potentially segregating for the transgenes.

Growth Requirement: none

Marker: kanamycin, hygromycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

GFP fluorescence in the nucleus and the cytoplasm of all cells. Nuclear fluorescent punctae in response to protein misfolding stress (e.g., heat shock).

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.1101/2020.08.30.274621
Transgene Description Promoter Reporter Marker
AL13Rb-HSP101 PCP-mGFP fusion and a PP7 reporter driven by the HSP101 promoter UBQ10; HSP101 GFP; PP7 kanamycin, hygromycin

Quality Control Comments

There is no quality control data for this stock.