NASC stock number: N72512
donor stock number: HsfA2-PP7-3
Resource Type: seed
Availability: available
Donors:- Krishna Niyogi
Donation Date: 04/06/2021
Date Released: 06/02/2021
Description:
Transgenic line. Col-0 transformed with the UPG plasmid (Addgene #161003) coding for a PCP-mGFP fusion driven by the Arabidopsis UBQ10 constitutive promoter and a second construct containing a PP7 reporter driven by the Arabidopsis HsfA2 promoter and a H2B-mScarlet nuclear marker (Addgene plasmid #161012). Hygromycin and kanamycin resistant. Potentially segregating for the transgenes.
Growth Requirement: none
Marker: kanamycin, hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
GFP fluorescence in the nucleus and the cytoplasm of all cells. Nuclear fluorescent punctae in response to protein misfolding stress (e.g., heat shock).
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1101/2020.08.30.274621 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| AL13Rb-HsfA2 | PCP-mGFP fusion driven by the Arabidopsis UBQ10 constitutive promoter and a second construct containing a PP7 reporter driven by the Arabidopsis HsfA2 promoter and a H2B-mScarlet nuclear marker | UBQ10; HsfA2 | GFP; PP7 |
Quality Control Comments
There is no quality control data for this stock.