Name / Stock Number: CS72561

other name: ago4-4/AGO4-D742A

donor stock number: AGO4::FLAG-AGO4(D742A) T5-4

NASC stock number: N72561

Resource Type: seed

Availability: available

Donors:
  • Michael Axtell

Donation Date: 07/09/2021

Date Released: 08/11/2021

Description:

Slicing-defective AGO4 driven by the native AGO4 promoter (pAGO4::FLAG-AGO4-D742A) expressed in the ago4-4 (CS72557) homozygous single mutant background. Mutagenesis of the catalytic motif of AGO4 was performed by overlapping extension PCR. Hygromycin resistant. Homozygous for the transgene.

Growth Requirement: none

Marker: hygromycin, kanamycin, BASTA

Background: Ws (Wassilewskija)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $13 / $104

Germplasm

2

partial recovery of AGO4-dependent small RNA accumulation

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.1111/tpj.13463 28002617
Transgene Description Promoter Reporter Marker
pGKB5 promoter trap T-DNA GUS kanamycin; BASTA
pAGO4::FLAG-AGO4-D742A slicing-defective AGO4 driven by the native AGO4 promoter AGO4 hygromycin

Quality Control Comments

There is no quality control data for this stock.