NASC stock number: N72589
donor stock number: TG8_pMDC162:168_5'delX:GUS
Resource Type: seed
Availability: available
Donors:- Dessiree Zerpa Catanho
- Ray Ming
Donation Date: 08/03/2021
Date Released: 09/24/2021
Description:
Transgenic line. Col-0 transformed with the pMDC162 vector containing a 5’ deletion (168 bp) on the 1 kb native promoter region of CpMDAR4 on the X chromosome for an experiment on the characterization of the CpMDAR4 promoter in Carica papaya. Kanamycin and hygromycin resistant. Segregating for the transgene. Donated seeds are T1 generation. Construct used to generate stock: CD3-2873 / Gene_art_1.
Growth Requirement: Use 0.5X MS selective medium containing 25 ug/ml Hygromycin for germination. Keep the plates in dark conditions at 4 C for at least 4 days, then place them in a growth chamber with a 16 h light and 8 h dark photoperiod at 23 C.
Marker: kanamycin, hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1007/s00497-022-00447-2 | 35920937 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| 168_5'delX:GUS | 1 kb native CpMDAR4-X promoter with a 5’ deletion (168 bp) in the pMDC162 vector | 5’del (168 bp) 1 kb CpMDAR4-X | GUS | kanamycin, hygromycin |
Quality Control Comments
There is no quality control data for this stock.