Name / Stock Number: CS72592

donor stock number: TG11_pMDC162:764_5'delY:GUS

NASC stock number: N72592

Resource Type: seed

Availability: available

Donors:
  • Ray Ming
  • Dessiree Zerpa Catanho

Donation Date: 08/03/2021

Date Released: 09/24/2021

Description:

Transgenic line. Col-0 transformed with a pMDC162 vector containing a 5' deletion (450 bp) on the 1 kb native promoter region of CpMDAR4 on the Y chromosome for an experiment on the characterization of the CpMDAR4 promoter in Carica papaya. Kanamycin and hygromycin resistant. Segregating for the transgene. Donated seeds are T1 generation. Construct used to generate stock: CD3-2876 / Gene_art_4.

Growth Requirement: Use 0.5X MS selective medium containing 25 ug/ml Hygromycin for germination. Keep the plates in dark conditions at 4 C for at least 4 days, then place them in a growth chamber with a 16 h light and 8 h dark photoperiod at 23 C.

Marker: kanamycin, hygromycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $13 / $104

Germplasm

2

Arabidopsis thaliana 3702

Additional Information

Transgene Description Promoter Reporter Marker
764_5'delX:GUS 1 kb native CpMDAR4-Y promoter with a 5’ deletion (450 bp) in the pMDC162 vector 5’del (450 bp) 1 kb CpMDAR4-Y GUS kanamycin, hygromycin

Quality Control Comments

There is no quality control data for this stock.