Name / Stock Number: CS72598

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NASC stock number: N72598

donor stock number: TG17_pMDC139:F:35S:MDAR4Y:GUS

Resource Type: seed

Availability: available

Donors:
  • Dessiree Zerpa Catanho
  • Ray Ming

Donation Date: 08/03/2021

Date Released: 09/24/2021

Description:

Transgenic line. Homozygous SALK_068667C plants transformed with the pMDC139 vector containing the CpMDAR4-Y allele for an experiment on the characterization of CpMDAR4 gene function. Kanamycin and hygromycin resistant. Segregating for the transgene. Donated seeds are T1 generation. Construct used to generate stock: CD3-2882 / MF1.

Growth Requirement: Use 0.5X MS selective medium containing 25 ug/ml Hygromycin and 1% Sucrose for germination. Keep the plates in dark conditions at 4 C for at least 4 days, then place them in a growth chamber with a 16 h light and 8 h dark photoperiod at 23 C.

Marker: kanamycin, hygromycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Genes

Gene Names Evidence
AT3G27820
  • MONODEHYDROASCORBATE REDUCTASE 4
  • ATMDAR4
  • MDAR4
    		•  polymorphism SIGnAL TAIR ARAPORT

    Germplasm

    2

    Polymorphisms

    Polymorphism Mutagen Genotype Inheritance Gene Evidence attribution
    SALK_068667.56.00.X T-DNA insertion homozygous
  • AT3G27820
    		• SIGnAL

    Arabidopsis thaliana 3702

    Additional Information

    DOI PubMed
    10.1007/s00497-022-00447-2 35920937
    Transgene Description Promoter Reporter Marker
    pROK2 T-DNA kanamycin
    35S:MDAR4Y:GUS CpMDAR4-Y allele in the pMDC139 vector CaMV 35S GUS kanamycin, hygromycin

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