Name / Stock Number: CS72601

donor stock number: TG20_pMDC139:I:35S:MDAR4Yh:GUS

NASC stock number: N72601

Resource Type: seed

Availability: available

Donors:
  • Ray Ming
  • Dessiree Zerpa Catanho

Donation Date: 08/03/2021

Date Released: 09/24/2021

Description:

Transgenic line. Homozygous SALK_068667C plants transformed with the pMDC139 vector containing an isoform of the CpMDAR4-Yh allele for an experiment on the characterization of CpMDAR4 gene function. Kanamycin and hygromycin resistant. Segregating for the transgene. Donated seeds are T1 generation. Construct used to generate stock: CD3-2885 / Yhiso.

Growth Requirement: Use 0.5X MS selective medium containing 25 ug/ml Hygromycin and 1% Sucrose for germination. Keep the plates in dark conditions at 4 C for at least 4 days, then place them in a growth chamber with a 16 h light and 8 h dark photoperiod at 23 C.

Marker: kanamycin, hygromycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $13 / $104

Germplasm

2

Arabidopsis thaliana 3702

Additional Information

Transgene Description Promoter Reporter Marker
pROK2 T-DNA kanamycin
35S:MDAR4Yh(I):GUS CpMDAR4-Yh isoform in the pMDC139 vector CaMV 35S GUS kanamycin, hygromycin

Quality Control Comments

There is no quality control data for this stock.