NASC stock number: N72608
donor stock number: KLR-EE-1
other name: D15 (KS999)
Resource Type: seed
Availability: available
Donors:- Patrick Krysan
Donation Date: 08/24/2021
Date Released: 10/08/2021
Description:
Independent transgenic line #1 containing the genetically encoded fluorescent biosensor Kinase Localization Reporter variant KLR-EE. KLR-EE consists of a bipartite modified nuclear localization signal (bNLS) with two phosphosites, a nuclear localization signal (NLS), the mNeonGreen fluorophore, a T2A "self-cleaving" Thosea asigna virus 2A peptide, the mRuby3 fluorophore, and an NLS derived from simian virus 40 (SV40). The two phosphosites within the bNLS have been mutagenized to phosphomimetic glutamic acids. This control construct does not contain a kinase docking domain. Primary transformants were identified with an epifluorescence microscope and seed was collected from plants exhibiting strong fluorescence. Transgene genotype unknown.
Growth Requirement: screen seedlings for fluorescence
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
red fluorescence present within the nuclei of cells, green fluorescence excluded from the nuclei of cells
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.3390/ijms21155350 | 32731410 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pKLR-EE | T-DNA construct expressing a fluorescent reporter for measuring kinase activity in plant cells. This version of the reporter is a control construct with no kinase docking domain and phosphomimetic mutations in the phosphoacceptor domain. | CaMV 35S | mNeonGreen/mRuby3 | n/a |
Quality Control Comments
There is no quality control data for this stock.