Name / Stock Number: CS72608

NASC stock number: N72608

donor stock number: KLR-EE-1

other name: D15 (KS999)

Resource Type: seed

Availability: available

Donors:
  • Patrick Krysan

Donation Date: 08/24/2021

Date Released: 10/08/2021

Description:

Independent transgenic line #1 containing the genetically encoded fluorescent biosensor Kinase Localization Reporter variant KLR-EE. KLR-EE consists of a bipartite modified nuclear localization signal (bNLS) with two phosphosites, a nuclear localization signal (NLS), the mNeonGreen fluorophore, a T2A "self-cleaving" Thosea asigna virus 2A peptide, the mRuby3 fluorophore, and an NLS derived from simian virus 40 (SV40). The two phosphosites within the bNLS have been mutagenized to phosphomimetic glutamic acids. This control construct does not contain a kinase docking domain. Primary transformants were identified with an epifluorescence microscope and seed was collected from plants exhibiting strong fluorescence. Transgene genotype unknown.

Growth Requirement: screen seedlings for fluorescence

Marker:

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $13 / $104

Germplasm

2

red fluorescence present within the nuclei of cells, green fluorescence excluded from the nuclei of cells

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.3390/ijms21155350 32731410
Transgene Description Promoter Reporter Marker
pKLR-EE T-DNA construct expressing a fluorescent reporter for measuring kinase activity in plant cells. This version of the reporter is a control construct with no kinase docking domain and phosphomimetic mutations in the phosphoacceptor domain. CaMV 35S mNeonGreen/mRuby3 n/a

Quality Control Comments

There is no quality control data for this stock.