Name / Stock Number: CS72613

NASC stock number: N72613

donor stock number: KLR-AA-MKP1-2

other name: AA06 (KS675)

Resource Type: seed

Availability: available

Donors:
  • Patrick Krysan

Donation Date: 08/24/2021

Date Released: 10/08/2021

Description:

Independent transgenic line #2 containing the genetically encoded fluorescent biosensor Kinase Localization Reporter KLR-AA-MKP1. KLR-AA-MKP1 consists of an 80 aa section of the Arabidopsis protein MAPK Phosphotase 1 (MKP1), which serves as a kinase docking domain, a bipartite modified nuclear localization signal (bNLS) with two phosphosites, a nuclear localization signal (NLS), the mNeonGreen fluorophore, a T2A "self-cleaving" Thosea asigna virus 2A peptide, the mRuby3 fluorophore, and an NLS derived from simian virus 40 (SV40). The two phosphosites within the bNLS have been mutagenized to non-phosphorylable alanines. Primary transformants were identified with an epifluorescence microscope and seed was collected from plants exhibiting strong fluorescence. Transgene genotype unknown.

Growth Requirement: screen seedlings for fluorescence

Marker:

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $13 / $104

Germplasm

2

red and green fluorescence present within the nuclei of cells

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.3390/ijms21155350 32731410
Transgene Description Promoter Reporter Marker
pKLR-AA-MKP1 T-DNA construct expressing a fluorescent reporter for measuring kinase activity in plant cells. This version of the reporter is a control consturct with a non-phosphorylable version of the phosphoacceptor domain and a kinase docking domain derived from a region of Arabidopsis MKP1. CaMV 35S mNeonGreen/mRuby3 n/a

Quality Control Comments

There is no quality control data for this stock.