Name / Stock Number: CS72615

NASC stock number: N72615

donor stock number: KLR-AP2C1-2

other name: D19 (KS928)

Resource Type: seed

Availability: available

Donors:
  • Patrick Krysan

Donation Date: 08/24/2021

Date Released: 10/08/2021

Description:

Independent transgenic line #2 containing the genetically encoded fluorescent biosensor Kinase Localization Reporter KLR-AP2C1. KLR-AP2C1 consists of a 35 aa section of the Arabidopsis protein PP2C-type phosphotase AP2C1, which serves as a kinase docking domain, a bipartite modified nuclear localization signal (bNLS) with two phosphosites, a nuclear localization signal (NLS), the mNeonGreen fluorophore, a T2A "self-cleaving" Thosea asigna virus 2A peptide, the mRuby3 fluorophore, and an NLS derived from simian virus 40 (SV40). Primary transformants were identified with an epifluorescence microscope and seed was collected from plants exhibiting strong fluorescence. Transgene genotype unknown.

Growth Requirement: screen seedlings for fluorescence

Marker:

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $13 / $104

Germplasm

2

red fluorescence present within the nuclei of cells, green fluorescence present within cells

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.3390/ijms21155350 32731410
Transgene Description Promoter Reporter Marker
pKLR-AP2C1 T-DNA construct expressing a fluorescent reporter for measuring kinase activity in plant cells. This version of the reporter is an active kinase reporter with a kinase docking domain derived from a region of Arabidopsis AP2C1 attached to a phosphorylable phosphoacceptor domain. CaMV 35S mNeonGreen/mRuby3 n/a

Quality Control Comments

There is no quality control data for this stock.