NASC stock number: N72615
donor stock number: KLR-AP2C1-2
other name: D19 (KS928)
Resource Type: seed
Availability: available
Donors:- Patrick Krysan
Donation Date: 08/24/2021
Date Released: 10/08/2021
Description:
Independent transgenic line #2 containing the genetically encoded fluorescent biosensor Kinase Localization Reporter KLR-AP2C1. KLR-AP2C1 consists of a 35 aa section of the Arabidopsis protein PP2C-type phosphotase AP2C1, which serves as a kinase docking domain, a bipartite modified nuclear localization signal (bNLS) with two phosphosites, a nuclear localization signal (NLS), the mNeonGreen fluorophore, a T2A "self-cleaving" Thosea asigna virus 2A peptide, the mRuby3 fluorophore, and an NLS derived from simian virus 40 (SV40). Primary transformants were identified with an epifluorescence microscope and seed was collected from plants exhibiting strong fluorescence. Transgene genotype unknown.
Growth Requirement: screen seedlings for fluorescence
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
red fluorescence present within the nuclei of cells, green fluorescence present within cells
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.3390/ijms21155350 | 32731410 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pKLR-AP2C1 | T-DNA construct expressing a fluorescent reporter for measuring kinase activity in plant cells. This version of the reporter is an active kinase reporter with a kinase docking domain derived from a region of Arabidopsis AP2C1 attached to a phosphorylable phosphoacceptor domain. | CaMV 35S | mNeonGreen/mRuby3 | n/a |
Quality Control Comments
There is no quality control data for this stock.