Name / Stock Number: CS72617

NASC stock number: N72617

donor stock number: KLR-AA-AP2C1-2

other name: D20 (KS947)

Resource Type: seed

Availability: available

  • Patrick Krysan

Donation Date: 08/24/2021

Date Released: 10/08/2021


Independent transgenic line #2 containing the genetically encoded fluorescent biosensor Kinase Localization Reporter KLR-AA-AP2C1. KLR-AA-AP2C1 consists of a 35 aa section of the Arabidopsis protein PP2C-type phosphotase AP2C1, which serves as a kinase docking domain, a bipartite modified nuclear localization signal (bNLS) with two phosphosites, a nuclear localization signal (NLS), the mNeonGreen fluorophore, a T2A "self-cleaving" Thosea asigna virus 2A peptide, the mRuby3 fluorophore, and an NLS derived from simian virus 40 (SV40). The two phosphosites within the bNLS have been mutagenized to non-phosphorylable alanines. Primary transformants were identified with an epifluorescence microscope and seed was collected from plants exhibiting strong fluorescence. Transgene genotype unknown.

Growth Requirement: screen seedlings for fluorescence


Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $13 / $104



red and green fluorescence present within the nuclei of cells

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.3390/ijms21155350 32731410
Transgene Description Promoter Reporter Marker
pKLR-AA-AP2C1 T-DNA construct expressing a fluorescent reporter for measuring kinase activity in plant cells. This version of the reporter is a control construct with a non-phosphorylable version of the phosphoacceptor domain and a kinase docking domain derived from a region of Arabidopsis AP2C1. CaMV 35S mNeonGreen/mRuby3 n/a

Quality Control Comments

There is no quality control data for this stock.