NASC stock number: N72723
donor stock number: AFB1-mCitrine#5
other name: MP3865
Resource Type: seed
Availability: available
Donors:- Mike Prigge
- Mark Estelle
Donation Date: 11/15/2021
Date Released: 01/07/2022
Description:
Transgenic line: pAFB1:gAFB1-mCitrine in the afb1-3 (SALK_070172; CS69650) background. Genomic fragment includes 958 bp upstream and 412 bp downstream from the coding region with a GARGGGGGA linker followed by mCitrine inserted at the native stop codon. BASTA resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: BASTA, kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
less YFP signal than line 7 (CS72724) but more than AFB1-Venus (PMID: 28472464)
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.7554/eLife.54740 | 32067636 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pAFB1:gAFB1-mCitrine | genomic fragment includes 958 bp upstream and 412 bp downstream from the coding region with a GARGGGGGA linker followed by mCitrine inserted at the native stop codon | pAFB1 | mCitrine | BASTA |
| pROK2 | T-DNA | kanamycin |
Quality Control Comments
There is no quality control data for this stock.