NASC stock number: N72729
donor stock number: AFB5-mCitrine#23
other name: MP3871
Resource Type: seed
Availability: available
Donors:- Mike Prigge
- Mark Estelle
Donation Date: 11/15/2021
Date Released: 01/07/2022
Description:
Transgenic line: pAFB5:gAFB5-mCitrine in the afb5-5 (SALK_110643; CS69643) background. Genomic fragment includes 1267 bp upstream and 1139 bp downstream from the coding region with a GARGGGGGA linker followed by mCitrine inserted at the native stop codon. BASTA resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: BASTA, kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
less YFP signal than line 9 (CS69659) but more signal than line 19 (CS69658)
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.7554/eLife.54740 | 32067636 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pAFB5:gAFB5-mCitrine | AFB5 genomic region between neighboring genes (from 1267 upstream from ATG to 1139 bp downstream of stop codon) fused to mCitrine | AFB5 | BASTA | |
| pROK2 | T-DNA | kanamycin |
Quality Control Comments
There is no quality control data for this stock.