Name / Stock Number: CS72743

NASC stock number: N72743

donor stock number: PLL12-comp

Resource Type: seed

Availability: available

Donors:
  • Avry Mudersbach
  • Charles Anderson

Donation Date: 11/17/2021

Date Released: 01/14/2022

Description:

PLL12 complementation line. The 2 kb PLL12 native promoter and PLL12 coding sequence were ligated and cloned into the pCR8/GW/TOPO entry vector, then LR cloned into vector pMDC110. The plasmid was transformed into pll12-1 (SAIL_1207_A07/CS878465) heterozygous plants and selected using methionine sulfoximine and hygromycin to obtain homozygous lines for both alleles.

Growth Requirement: none

Marker: BASTA, hygromycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.1093/plcell/koab161 34109391
Transgene Description Promoter Reporter Marker
pDAP101 T-DNA BASTA
PLL12pro:PLL12 PLL12 complementation construct PLL12pro hygromycin

Quality Control Comments

There is no quality control data for this stock.