NASC stock number: N72757
donor stock number: YFP-HAM2 in h124
other name: ham1;2;4 [pHAM2::YFP-HAM2]
Resource Type: seed
Availability: available
Donors:- Elliot Meyerowitz
Donation Date: 11/29/2021
Date Released: 02/04/2022
Description:
ham1;2;4 triple mutant (CS67136) complemented with the pHAM2::YFP-HAM2 transgene. The YFP variant YPET was amplified and cloned in front of HAM2 cDNA in-frame to generate the YFP-HAM2 fragment. The 3,122 bp HAM2 promoter was then amplified and cloned in front of YFP-HAM2, and the 1,140 bp HAM2 3’ untranslated sequence was amplified and cloned 3’ of YFP-HAM2. The whole fused DNA fragment was cloned into the binary vector pMOA34 and introduced into ham1;2;4 plants (CS67136) using the floral dip method. Hygromycin selection. Homozygous for the transgene.
Growth Requirement: hygromycin selection
Marker: BASTA, hygromycin, kanamycin
Background: Col (Columbia); Ler (Landsberg erecta); Ws (Wassilewskija)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1038/nature13853 | 25363783 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| GTDs | gene trap transposon | GUS | kanamycin | |
| pGKB5 | promoter trap T-DNA | GUS | BASTA, kanamycin | |
| pHAM2::YFP-HAM2 | HAM2 reporter construct | pHAM2 | YFP | hygromycin |
| pROK2 | T-DNA | kanamycin |
Quality Control Comments
There is no quality control data for this stock.