NASC stock number: N72761
donor stock number: mddcc
Resource Type: seed
Availability: available
Donors:- Li He
Donation Date: 04/04/2022
Date Released: 04/13/2022
Description:
Quintuple mutant (met1-8, drm1-2, drm2-2, cmt3-11, cmt2-3) generated by CRISPR/Cas9-mediated mutation of MET1 directly in the ddcc quadruple mutant background. Two sgRNAs were designed to target the first exon of the MET1 gene and the CRISPR-Cas9 construct was transformed into the ddcc mutant background. Then, heterozygous met1 mutants (met1/+) without the CRISPR-Cas9 construct were isolated from the mutated T2 populations in the ddcc background. Deposited seeds are the progenies of met1/+ drm1 drm2 cmt3 cmt2 plants and were used to isolate mddcc DNA methylation-free quintuple mutant plants.
Growth Requirement: none
Marker: kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Quintuple homozygous plants exhibit extreme growth retardation and small size, display a suite of severe developmental defects, and do not undergo floral transition.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1038/s41467-022-28940-2 | 35288562 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pROK2 | T-DNA | kanamycin |
Quality Control Comments
There is no quality control data for this stock.