ABRC
NASC stock number: N73387
donor stock number: 555
other name: Bxb1_Target
Resource Type: seed
Availability: available
Donors:- Jennifer Nemhauser
- Sarah Guiziou
Donation Date: 02/03/2023
Date Released: 03/06/2023
Description:
Stable transgenic line expressing the Bxb1 serine integrase target construct, consisting of the pUBQ10 constitutive promoter flanked by Bxb1 integrase sites positioned between two reporter genes: the mScarlet and mTurquoise2 (mTurq) fluorescent proteins. Col-0 plants were transformed using the floral dip method. Kanamycin resistant. Segregating for the transgene.
Growth Requirement: none
Marker: kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
Ploidy: 2
Phenotype: mTurq expression in roots and leaves before integrase switch, mScarlet expression after integrase switch
Taxon: Arabidopsis thaliana 3702
Publications
| DOI | PubMed |
|---|---|
| https://doi.org/10.1038/s41467-023-37607-5 | 37012288 |
Transgenes
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| Bxb1_integrase_target | pUBQ10 constitutive promoter flanked by Bxb1 integrase sites positioned between two reporter genes: the mScarlet and mTurquoise2 (mTurq) fluorescent proteins: tRBCS-mScarlet-attPBxb1-pUBQ10-attBBxb1-mTurq-tUBQ10 | pUBQ10 | mTurquoise2; mScarlet | kanamycin |
Quality Control Comments
There is no quality control data for this stock.