Name / Stock Number: CS73389

NASC stock number: N73389

donor stock number: 444 L1P97 T2P10

other name: PhiC31_Target+pGATA23::PhiC31:DST

Resource Type: seed

Availability: available

  • Sarah Guiziou
  • Jennifer Nemhauser

Donation Date: 02/03/2023

Date Released: 03/06/2023


Stable transgenic PhiC31 serine integrase target line (CS73386) also expressing a PhiC31 integrase with a DST tag (destabilization RNA tag) under the pGATA23 promoter. Col-0 plants were transformed using the floral dip method. Kanamycin/hygromycin resistant. Segregating for the target construct, homozygous for the integrase construct.

Growth Requirement: none

Marker: kanamycin, hygromycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $13 / $104



Most seedlings should have mScarlet expression only in lateral roots, as integrase switch occurs in early stage of lateral roots.

Arabidopsis thaliana 3702

Additional Information

Transgene Description Promoter Reporter Marker
PhiC31_integrase_target pUBQ10 constitutive promoter flanked by PhiC31 integrase sites positioned between two reporter genes: the mScarlet and mTurquoise2 (mTurq) fluorescent proteins: tRBCS-mScarlet-attPPhiC31-pUBQ10-attBPhiC31-mTurq-tUBQ10 pUBQ10 mTurquoise2; mScarlet kanamycin
pGATA23::PhiC31:DST integrase construct: PhiC31 integrase with a DST tag under the pGATA23 promoter (pGATA23::PhiC31:DST:tUBQ10) pGATA23 hygromycin

Quality Control Comments

There is no quality control data for this stock.