NASC stock number: N73560
donor stock number: pLH13 in as2-7D
Resource Type: seed
Availability: available
Donors:- Adrienne Roeder
- Lilan Hong
Donation Date: 03/25/2024
Date Released: 04/03/2024
Description:
Transgenic line carrying fluorescent membrane marker pLH13 (p35S::mCitrine-RCI2A) in an as2-7D mutant background. The as2-7D allele has a G to A mutation at base 1,484 upstream of the ATG translational start site of AT1G65620. The transgene pLH13 consists of mCitrine tagged to RARE-COLD-INDUCIBLE 2A (RCI2A) protein driven by the CaMV 35S promoter. The membrane marker silences frequently, and hence requires screening in each generation.
Growth Requirement: none
Marker:
Background:
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
The sepals exhibit folds and outgrowths on the outer epidermis, while the inner epidermis is smooth. The cotyledons exhibit upward leaf curving. The older vegetative leaves show some degree of upward curving as well. The mutant also exhibits late flowering.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1101/2023.07.22.549953 | 37546730 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| p35S::mCitrine-RCI2A | yellow flourescent protein based membrane marker (pLH13, mCitrine tagged to RARE-COLD-INDUCIBLE 2A (RCI2A) protein) driven by CaMV 35S promoter |
Quality Control Comments
There is no quality control data for this stock.