NASC stock number: N73563
donor stock number: ATML1p::LGO pLH13
Resource Type: seed
Availability: available
Donors:- Adrienne Roeder
Donation Date: 03/25/2024
Date Released: 04/03/2024
Description:
Transgenic line carrying the ATML1p:LGO transgene for over-expression of Loss of Giant cells from Organs (LGO) and the fluorescent membrane marker pLH13 (p35S::mCitrine-RCI2A) which consists of mCitrine tagged to RARE-COLD-INDUCIBLE 2A (RCI2A) protein driven by the CaMV 35S promoter. The membrane marker silences frequently, and hence requires screening in each generation.
Growth Requirement: none
Marker:
Background:
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
The epidermis has a greater number of highly endoreduplicated cells. The sepals have less curvature and the leaves are wrinkled. They also have mCitrine expression in the membrane.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1101/2023.11.11.566685 | 38014347 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| ATML1p::LGO | Over-expression of the gene LOSS of GIANT Cells from Organs under epidermial-specific promoter | ATML1 | BASTA | |
| p35S::mCitrine-RCI2A | yellow flourescent protein based membrane marker (pLH13, mCitrine tagged to RARE-COLD-INDUCIBLE 2A (RCI2A) protein) driven by CaMV 35S promoter |
Quality Control Comments
There is no quality control data for this stock.