Name / Stock Number: CS73564

NASC stock number: N73564

donor stock number: ATML1p::LGO ftsh4-5 pLH13

Resource Type: seed

Availability: available

Donors:
  • Adrienne Roeder

Donation Date: 03/25/2024

Date Released: 04/03/2024

Description:

Transgenic line carrying the ATML1p:LGO transgene for overexpression of Loss of Giant cells from Organs (LGO) and the fluorescent membrane marker pLH13 (p35S::mCitrine-RCI2A) in an ftsh4-5 mutant background. pLH13 consists of mCitrine tagged to RARE-COLD-INDUCIBLE 2A (RCI2A) protein driven by the CaMV 35S promoter. The membrane marker silences frequently, and hence requires screening in each generation.

Growth Requirement: none

Marker:

Background:

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

The epidermis has a greater number of highly endoreduplicated cells. The sepals have less curvature and have variable size and shape. The leaves are serrated and wrinkled. They also have mCitrine expression in the membrane.

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.1101/2023.11.11.566685 38014347
Transgene Description Promoter Reporter Marker
ATML1p::LGO Over-expression of the gene LOSS of GIANT Cells from Organs under epidermial-specific promoter ATML1 BASTA
p35S::mCitrine-RCI2A yellow flourescent protein based membrane marker (pLH13, mCitrine tagged to RARE-COLD-INDUCIBLE 2A (RCI2A) protein) driven by CaMV 35S promoter

Quality Control Comments

There is no quality control data for this stock.