Name / Stock Number: CS73702

NASC stock number: N73702

donor stock number: L1T10

Resource Type: seed

Availability: available

Donors:
  • Jennifer Nemhauser
  • Cassandra Maranas

Donation Date: 06/25/2024

Date Released: 06/27/2024

Description:

Transgenic line expressing integrase-based, history-dependent recorder of gene expression, PhiC31 then Bxb1 history dependent integrase target with pUBQ10. Initial fluorescent expression (expressed with pUBQ10) is BFP switching to RFP with the first PhiC31 integrase switch and then from RFP to GFP with the second Bxb1 integrase switch. The 'out of order' Bxb1 integrase switch (occurring before PhiC31 switching) causes a loss of BFP fluorescence.

Growth Requirement: none

Marker:

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.1101/2024.06.04.597320
Transgene Description Promoter Reporter Marker
PhiC31 then Bxb1 history dependent integrase target with pUBQ10 tUBQ1-NeonGreen-attPBxb1-pUBQ10-attPPhiC31-ERtag-mtagBFP2-tUBQ10-tRBCS-NLS-mCherry-attBBxb1-attBPhiC31

Quality Control Comments

There is no quality control data for this stock.