NASC stock number: N73703
donor stock number: L1T11
Resource Type: seed
Availability: available
Donors:- Jennifer Nemhauser
- Cassandra Maranas
Donation Date: 06/25/2024
Date Released: 06/27/2024
Description:
Transgenic line expressing integrase-based, history-dependent recorder of gene expression, Bxb1 then PhiC31 history dependent integrase target with p35S. Initial fluorescent expression (expressed with p35S) is BFP switching to RFP with the first Bxb1 integrase switch and then from RFP to GFP with the second PhiC31 integrase switch. The 'out of order' PhiC31 integrase switch (occurring before Bxb1 switching) causes a loss of BFP fluorescence.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1101/2024.06.04.597320 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| Bxb1 then PhiC31 history dependent integrase target with p35S | tUBQ1-NeonGreen-attPPhiC31-p35S-attPBxb1-ERtag-mtagBFP2-tUBQ10-tRBCS-NLS-mCherry-attBPhiC31-attBBxb1 |
Quality Control Comments
There is no quality control data for this stock.