NASC stock number: N74154
donor stock number: KasI inducible
Resource Type: seed
Availability: available
Donors:- Moira Rodriguez
Donation Date: 12/15/2025
Date Released: 12/17/2025
Description:
transgenic line, contains a nuclear T-DNA insertion expression cassette containing an inducible restriction enzyme (KasI) with mitochondrial targeting; inducer is B-estradiol; plant selectable marker is hygromycin; KasI has 35 target sites in the BK010421 A. thaliana mitochondrial genome
Growth Requirement:
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| KasI | KasI restriction enzyme coding sequence, including an mCBC intron to prevent expression during cloning. Plant expression driven by an estrogen receptor-based chemical-inducible system (XVE). KasI is targeted to the mitochondria via a fragment of AOX1A. T-DNA sequence also includes a Hygromycin resistance marker. | G10-90 | hygromycin |
Quality Control Comments
There is no quality control data for this stock.