ABRC
NASC stock number: N74415
donor stock number: pWRKY70:ABL.1
other name: 4197
other name: Salicylic acid-responsive fungal bioluminescent line 01
Resource Type: seed
Availability: available
Donors:- Mike Karampelias
Donation Date: 09/09/2026
Date Released: 09/10/2026
Description:
transgenic line for non-invasive imaging of defense responses, biouminescent salicylic acid reporter; the masterline was transformed witha plasmid carrying hygromycin resistance cassette driven by pNos promoter and ocs terminator from A. tumefaciens and nnLuz_v423 under the control of WRKY70 promoter (2060 bp upstream of A. thaliana WRKY70 start codon) and terminator (500 bp downstream A. thaliana WRKY70 stop codon)
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
Ploidy: 2
Phenotype: these plants produce fungal autonomous bioluminescence driven by the WRKY70 promoter
Taxon: Arabidopsis thaliana 3702
Publications
| DOI | PubMed |
|---|---|
| 10.1038/s41467-026-70075-1 | 41826305 |
Transgenes
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| master construct | pNOS::KanR::tOCS, p35S::nnHispS::tOCS, pCmYLCV::npgA::tATP, p35SnnCPH::tOCS, pFMV::nnH3H::tNOS | kanamycin | ||
| pWRKY70:ABL.1 | pNOS::HygR::ocsT, pWRKY70::nnLuz::tWRKY70 | WRKY70 | hygromycin |
Quality Control Comments
There is no quality control data for this stock.