donor stock number: DsG1
NASC stock number: N8049
Resource Type: seed
Availability: available
Donors:- Venkatesan Sundaresan
- Robert Martienssen
Donation Date: 03/01/1996
Date Released: 03/01/1996
Description:
contains maize Dissociation (Ds) transposable element; gene trap (exon trap, intron trap) line; kanamycin resistant (NPTII); beta-glucuronidase (GUS) with intron splice acceptor sites in all three frames, no promoter; naphthalene acetamide (NAM) sensitive (contains indole acetic acid hydrolase, IAAH); crosses to Ac lines (CS8043, CS8044, and CS8045) induce transposition of Ds; selection for kanamycin resistance and NAM insensitivity in F2 generation results in transposed lines lacking Ac, and favors transpositions not closely linked to the donor Ds site; expression of GUS may occur if 5-prime end of GUS is transposed into an intron or in-frame in an exon; introduced into plants by Agrobacterium transformation - contains T-DNA borders.
Growth Requirement: none
Marker: kanamycin
Background: Ler (Landsberg erecta)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
transposon insertion
Arabidopsis thaliana 3702
Additional Information
DOI | PubMed |
---|---|
10.1101/gad.9.14.1797 | 7622040 |
Transgene | Description | Promoter | Reporter | Marker |
---|---|---|---|---|
GTDs | gene trap transposon | GUS | kanamycin |
Quality Control Comments
There is no quality control data for this stock.