donor stock number: E43 (W)
NASC stock number: N8129
Resource Type: seed
Availability: available
Donors:- Barbara Baker
Donation Date: 06/20/1996
Date Released: 06/20/1996
Description:
transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the existing line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in W orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements.
Growth Requirement: none
Marker: kanamycin
Background: No (Nossen)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
transposon insertion
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1046/j.1365-313X.1995.7040687.x | 7742862 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| lox-Dslox | transposon |
Quality Control Comments
There is no quality control data for this stock.