NASC stock number: N841758
Resource Type: seed
Availability: available
Donors:- Syngenta, USA
Donation Date: 03/01/2004
Date Released: 06/04/2004
Description:
T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pCSA110, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; homozygous for qrt (quartet, pollen tetrad defect) mutation; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.
Growth Requirement: none
Marker: BASTA
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
DOI | PubMed |
---|---|
10.1105/tpc.004630 | 12468722 |
Transgene | Description | Promoter | Reporter | Marker |
---|---|---|---|---|
pCSA110 | T-DNA | LAT52 | GUS | BASTA |
Quality Control Comments
Comment | Commented by | Date | |
---|---|---|---|
new T-DNA insertions have been identified in this stock by TDNA-Seq, a method for next-generation sequencing performed in J. Ecker lab at SALK Institute | ABRC | 03/10/2015 | To view detailed data, click here |
Researcher reported zero germination | ABRC | 07/06/2018 | To view detailed data, click here |
Germination was found to be 20%; this stock will be not available until the stock can be regrown. | ABRC | 07/19/2018 | To view detailed data, click here |
Previous low germination stock was replaced with a stock that the germination was found to be 100%. | ABRC | 06/17/2019 | To view detailed data, click here |